circRNA basic information
circBase ID: hsa_circ_0036683
Name: hsa_circ_ABHD2
Synonym: circ‐36683 / circ-36683
Host Gene: ABHD2
Genomic location(hg19): chr15:89656955-89698765:+
Genomic location(hg38): chr15:89113724-89155534:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; paracarcinoma tissues; xenograft tumor tissue

Cell lines:

A549; H1975; H1299; BEAS-2B

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: CDK2AP2
Associated microRNA: miR-4664-3p
Biological function: Suppresses proliferation and migration; induces G0/G1 phase arrest; suppresses xenograft tumor growth and angiogenesis
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-4664-3p to upregulate CDK2AP2, modulating p53/p21/p27 and CDK2/cyclin E1 expression
Biological pathway or process:

proliferation (inhibits); migration (inhibits); cell cycle (inhibits); apoptosis (unclear); p53 signaling (promotes); angiogenesis (inhibits); ceRNA regulation (promotes)

Detected method:
Q
H
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Wound Healing Assay; Transwell Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation

Clinical significance:

circ-36683 expression is decreased in NSCLC tissues vs paracarcinoma and negatively correlates with miR-4664-3p expression

Description:

hsa_circ_0036683 (circ-36683), a circRNA derived from ABHD2, is down-regulated in NSCLC tissues and lung cancer cell lines. It mainly localizes to the cytoplasm and suppresses NSCLC cell proliferation, migration, and xenograft growth by sponging miR-4664-3p to relieve repression of CDK2AP2, thereby promoting p53/p21/p27 and inhibiting CDK2/cyclin E1 to induce G0/G1 arrest.

Confidence score:

0.8521

Other information
Title:

Novel circular RNA hsa_circ_0036683 suppresses proliferation and migration by mediating the miR-4664-3p/CDK2AP2 axis in non-small cell lung cancer.

Journal: Thoracic cancer
Published: 2024
PubMed ID: 39113208
Study type:

combined biological and clinical study

Data availability: All data generated during this study are included either in the article or in the additional files.
Code availability: -