circRNA basic information
circBase ID: hsa_circ_0006677
Name: hsa_circ_WDR78
Synonym: -
Host Gene: WDR78
Genomic location(hg19): chr1:67356836-67371058:-
Genomic location(hg38): chr1:66891153-66905375:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor samples; corresponding non-tumor samples; xenograft tumors
Cell lines:

16 HBE; H1299; A549; HCC827; H460
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: SATB2, Ago2, MEK, ERK
Associated microRNA: miR-1245a
Biological function: Suppresses NSCLC malignancy and stemness by inhibiting proliferation, migration, invasion, stemness and inducing apoptosis; decreases xenograft tumor growth.
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-1245a to upregulate SATB2; modulates MEK/ERK pathway activity via the miR-1245a/SATB2 axis.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); stemness (inhibits); ERK (inhibits); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis; Microarray
Clinical significance:

-
Description:

In NSCLC, circ_0006677 is down-regulated and acts as a tumor suppressor. It sponges miR-1245a to increase SATB2 expression and thereby inhibits malignant phenotypes (proliferation, migration, invasion, stemness) while promoting apoptosis, partly via inhibiting MEK/ERK signaling.
Confidence score:

0.8254
Other information
Title:

Hsa_circ_0006677 regulates special AT-rich binding protein-2-mediated tumor-suppressive effect via functioning as a miR-1245a sponge in non-small cell lung cancer.

Journal: Bioengineered
Published: 2022
PubMed ID: 35081869
Study type:

combined biological and clinical study
Data availability: GSE158695; GSE112214
Code availability: -