| Expression pattern: |
DN |
| Associated gene: |
Annexin A2 (ANXA2) |
| Associated microRNA: |
- |
| Biological function: |
circSLC8A1 maintains tubular epithelial phenotype and limits tubular partial EMT, tubular dedifferentiation, fibrogenesis, renal fibrosis and ANXA2 protein stabilization; loss of circSLC8A1 promotes partial EMT and fibrosis, while overexpression reduces fibrosis in UUO and UIRI mouse models. |
| Molecular mechanism: |
circSLC8A1 directly binds Domain IV of ANXA2 and promotes ANXA2 polyubiquitination and ubiquitin-proteasome-dependent degradation; circSLC8A1 deficiency stabilizes ANXA2 protein, driving tubular partial EMT and fibrosis. |
| Biological pathway or process: |
EMT (inhibits); fibrosis (inhibits); ubiquitination (promotes); other pathway/process (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; H&E Staining; Bioinformatics Analysis; Cohort Study |
| Clinical significance: |
circSLC8A1 is deficient in CKD and lower expression is linked to more severe disease, renal fibrosis, worsening kidney function, no resolution of proteinuria over time, and worse clinical prognosis; it may be a diagnostic and prognostic indicator. |
| Description: |
circSLC8A1/circSlc8a1 is a kidney-enriched circRNA downregulated in human CKD, TGF-beta-induced tubular epithelial cells, and UUO/UIRI renal fibrosis mouse models. Its loss promotes tubular partial EMT and fibrogenesis by stabilizing ANXA2 protein, whereas restoration of circSlc8a1 attenuates renal fibrosis in vivo. Clinically, lower circSLC8A1 expression correlates with worse kidney function, fibrosis severity and poor prognosis. |
| Confidence score: |
0.8892 |