| Expression pattern: |
DN |
| Associated gene: |
MYCL rs1038259890, MYCL c.247 G>A, MYCL p.Gly83Ser, KDM4C |
| Associated microRNA: |
- |
| Biological function: |
Functions as a tumor suppressor in B[a]P-induced malignant transformed 16HBE cells; its downregulation promotes proliferation and migration, accelerates MYCL c.247 G>A mutagenesis, and contributes to malignant transformation. |
| Molecular mechanism: |
circ_0001839 downregulation accelerates acquisition of the MYCL rs1038259890 C>T mutation during prolonged B[a]P exposure, compromising genomic integrity and facilitating clonal selection of the MYCL p.Gly83Ser driver mutation. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNA-seq; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Transfection; EdU Staining; Wound Healing Assay |
| Clinical significance: |
Potential biomarker and therapeutic/preventive target for pollution-driven or environmentally induced lung cancers; potential early biomarker for assessing genotoxic effects of chemical carcinogen exposure. |
| Description: |
circ_0001839 is a KDM4C-derived human circRNA that is downregulated during B[a]P-induced malignant transformation and in lung cancer cell lines. It acts as a tumor suppressor: overexpression inhibits proliferation and migration, whereas knockdown promotes these malignant phenotypes and accelerates acquisition of the oncogenic MYCL rs1038259890/p.Gly83Ser mutation. The study proposes circ_0001839 as a potential early biomarker and preventive or therapeutic target for environmentally induced lung carcinogenesis. |
| Confidence score: |
0.6628 |