circRNA basic information
circBase ID: hsa_circ_0006404
Name: hsa_circ_FOXO3
Synonym: circular RNA FOXO3
Host Gene: FOXO3
Genomic location(hg19): chr6:108984657-108986092:+
Genomic location(hg38): chr6:108663454-108664889:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0011962
MONDO name: endometrial cancer
Disease details: endometrial cancer / EC
Disease DO ID:
1380
Disease MeSH ID:
-
Disease NCIt ID:
C27815
Disease ICD11 ID:
-
Disease OMIM ID:
608089
Species: Human
Species details: Homo sapiens
Tissue specimen:

EC tissues; matched para-cancerous normal tissues; adjacent normal tissues; tumor tissues

Cell lines:

Ishikawa; HEC-1A; HEC-1B; HEC-251; RL95-2; T-HESC

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: HDAC4
Associated microRNA: miR-29a-3p
Biological function: Promotes EC cell proliferation, cell cycle progression, migration, invasion, and tumor growth, and inhibits apoptosis; circ_FOXO3 knockdown suppresses EC progression in vitro and in vivo.
Molecular mechanism: circ_FOXO3 functions as a ceRNA/miRNA sponge for miR-29a-3p, thereby upregulating HDAC4 and promoting EC progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); metastasis (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Cohort Study; Bioinformatics Analysis

Clinical significance:

High circ_FOXO3 expression was associated with shorter overall survival, advanced FIGO stage, severer histological grade, and more lymphatic metastasis; circ_FOXO3 may be a promising therapeutic target in EC.

Description:

circ_FOXO3 is up-regulated in human endometrial carcinoma tissues and cell lines and is associated with poor overall survival and aggressive clinicopathological features. Functionally, circ_FOXO3 promotes EC proliferation, migration, invasion, cell-cycle progression, and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a miR-29a-3p sponge to increase HDAC4 expression through the circ_FOXO3/miR-29a-3p/HDAC4 axis.

Confidence score:

0.7412

Other information
Title:

Circular RNA FOXO3 regulates endometrial carcinoma progression through the microRNA-29a-3p/HDAC4 axis.

Journal: American journal of translational research
Published: 2022
PubMed ID: 36628205
Study type:

combined biological and clinical study

Data availability: -
Code availability: -