circRNA basic information
circBase ID: hsa_circ_0032704
Name: hsa_circ_TTLL5
Synonym: circTTLL5 / circular RNA tubulin tyrosine ligase like 5
Host Gene: TTLL5
Genomic location(hg19): chr14:76173360-76187046:+
Genomic location(hg38): chr14:75707017-75720703:+
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

primary HCC tissues; adjacent normal tissues; blood; serum; HCC tissues; matched normal tissues
Cell lines:

HuH-7; HCCLM3; THLE-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KIAA1522
Associated microRNA: miR-136-5p
Biological function: Exosomal circTTLL5 promotes HCC cell proliferation, migration, invasion, EMT, metastasis and tumor growth, and inhibits apoptosis.
Molecular mechanism: Exosome-mediated transfer of circTTLL5 acts as a ceRNA by sponging miR-136-5p, thereby indirectly upregulating KIAA1522 to promote HCC progression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model
Clinical significance:

Blockage of the exosome-mediated transfer of circTTLL5 might be a therapeutic target for HCC.
Description:

circTTLL5 (hsa_circ_0032704), derived from TTLL5, is up-regulated in HCC tissues, cells and exosomes. Exosome-mediated circTTLL5 transfer promotes HCC proliferation, EMT, migration, invasion, metastasis and xenograft tumor growth while suppressing apoptosis. Mechanistically, circTTLL5 functions as a ceRNA that sponges miR-136-5p to relieve repression of KIAA1522, suggesting a potential therapeutic target in HCC.
Confidence score:

0.78
Other information
Title:

Exosome-mediated circTTLL5 transfer promotes hepatocellular carcinoma malignant progression through miR-136-5p/KIAA1522 axis.

Journal: Pathology, research and practice
Published: 2023
PubMed ID: 36528986
Study type:

combined biological and clinical study
Data availability: GSE78520; GSE94508; GSE97332; TCGA
Code availability: -