| Expression pattern: |
UP |
| Associated gene: |
ROS1 |
| Associated microRNA: |
miR-101-3p |
| Biological function: |
promotes glioblastoma cell proliferation and motility (migration/metastasis) and tumor growth |
| Molecular mechanism: |
acts as a miRNA sponge for miR-101-3p to regulate ROS1 expression; ROS1-circENTPD7 feedback |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis |
| Clinical significance: |
glioblastoma patients had a poor overall survival when circENTPD7 expression levels were high; high circENTPD7 correlated with advanced classification and tumor size |
| Description: |
circENTPD7 is up-regulated in glioblastoma tissues/cells and promotes proliferation and motility. Mechanistically, it functions as a ceRNA by sponging miR-101-3p to increase ROS1 expression, and its knockdown suppresses xenograft tumor growth. High circENTPD7 expression is associated with shorter overall survival in glioblastoma patients. |
| Confidence score: |
0.8537 |