| Expression pattern: |
UP |
| Associated gene: |
HK2, Ago2 |
| Associated microRNA: |
miR-145 |
| Biological function: |
promotes glycolysis and lactate generation; promotes NSCLC cell proliferation, colony formation, migration and metastasis ability; inhibits apoptosis; promotes Treg cell infiltration and immune evasion; enhances tumor growth in vivo |
| Molecular mechanism: |
circRUNX1 functions as a miR-145 sponge to derepress HK2, thereby promoting glycolysis, lactate production, the Warburg effect, Treg infiltration and immune evasion in NSCLC |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); metastasis (promotes); apoptosis (inhibits); immune regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Survival Analysis; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
high circRUNX1 expression is associated with advanced tumor stage, lymph node metastasis, tumor size, distant metastasis and worse prognosis in NSCLC patients |
| Description: |
circRUNX1 is up-regulated in NSCLC tissues and cells and high expression is associated with advanced clinicopathological features and poor prognosis. Mechanistically, circRUNX1 sponges miR-145 to increase HK2 expression, enhancing glycolysis, lactate production and the Warburg effect. This metabolic reprogramming promotes Treg infiltration and immune evasion while supporting NSCLC proliferation, migration and tumor growth. |
| Confidence score: |
0.8661 |