| Expression pattern: |
UP |
| Associated gene: |
GSPT1 |
| Associated microRNA: |
miR-498 |
| Biological function: |
promotes proliferation; promotes invasion; promotes migration; promotes tumorigenesis |
| Molecular mechanism: |
hsa_circ_0001944 acts as a miRNA sponge for miR-498 to increase GSPT1 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); invasion (promotes); migration (promotes); tumorigenesis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; ROC Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Microarray |
| Clinical significance: |
ROC curves demonstrated that hsa_circ_0001944 may be a potential biomarker of gastric cancer (AUC = 90.5%); high hsa_circ_0001944 expression was correlated with poor prognostic factors (including poor differentiation and advanced TNM stage). |
| Description: |
hsa_circ_0001944 (circFIRRE) is up-regulated in gastric cancer and functions as an oncogenic circRNA. It mainly localizes to the cytoplasm and promotes proliferation, invasion, migration, and in vivo tumor growth by sponging miR-498 to increase GSPT1 expression, forming the hsa_circ_0001944/miR-498/GSPT1 axis. |
| Confidence score: |
0.8792 |