| Expression pattern: |
UP |
| Associated gene: |
GLUT1 |
| Associated microRNA: |
miR-150-5p |
| Biological function: |
promotes glycometabolism, proliferation and metastasis in NSCLC; circARHGAP10 knockdown suppresses tumor growth and lung metastasis |
| Molecular mechanism: |
Acts as a miR-150-5p sponge to promote GLUT1 expression; affects glucose metabolism and EMT-related markers |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence) |
| Clinical significance: |
High circARHGAP10 expression is correlated with poorer overall survival in NSCLC patients |
| Description: |
circARHGAP10 (hsa_circ_0008975), derived from ARHGAP10, is upregulated in NSCLC tissues and cell lines and is mainly localized in the cytoplasm. It promotes NSCLC cell proliferation, glycolytic metabolism, EMT-associated metastasis by sponging miR-150-5p to increase GLUT1, and high expression is associated with poor overall survival. |
| Confidence score: |
0.8155 |