circRNA basic information
circBase ID: hsa_circ_0079530
Name: hsa_circ_Twist1
Synonym: circ_0079530
Host Gene: Twist1
Genomic location(hg19): chr7:19155090-19155754:-
Genomic location(hg38): chr7:19115467-19116131:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tumor tissue; adjacent non-tumor tissue (ANT)
Cell lines:

H2170; A549; MRC-5
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AQP4
Associated microRNA: miR-409-3p
Biological function: promotes cell proliferation, migration, and invasion, and reduces radiosensitivity (circ_0079530 knockdown shows opposite effects); promotes tumor growth in vivo
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-409-3p to regulate AQP4 expression, affecting NSCLC progression and radiosensitivity
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); radioresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; EdU Staining; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Clinical Sample Validation; In Vivo Animal Model; Western Blot
Clinical significance:

Circ_0079530 expression was higher in radioresistant samples than in radiosensitive tissues
Description:

This study reports that hsa_circ_0079530 (circ_0079530), derived from Twist1, is up-regulated in NSCLC tissues and especially in radioresistant samples. Functionally, circ_0079530 promotes NSCLC cell proliferation, migration/invasion and tumor growth, and decreases radiosensitivity. Mechanistically, it sponges miR-409-3p to upregulate AQP4, forming a circ_0079530/miR-409-3p/AQP4 axis that drives malignancy and radioresistance.
Confidence score:

0.7979
Other information
Title:

Hsa_circ_0079530/AQP4 Axis Is Related to Non-Small Cell Lung Cancer Development and Radiosensitivity.

Journal: Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia
Published: 2022
PubMed ID: 35896371
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -