| Expression pattern: |
UP |
| Associated gene: |
X-linked inhibitor of apoptosis / XIAP |
| Associated microRNA: |
miR-142-3p |
| Biological function: |
Promotes colorectal cancer cell proliferation, migration and invasion, and inhibits apoptosis; associated with enlarged tumour volume and advanced TNM stage. |
| Molecular mechanism: |
Circ_0006174 acts as a ceRNA by sponging miR-142-3p, thereby positively regulating XIAP expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; BrdU; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High expression was associated with enlarged tumour volume and advanced TNM stage; the study suggests potential diagnostic biomarker and therapeutic target value. |
| Description: |
Circ_0006174 is an up-regulated RAD23B-derived circRNA in human colorectal cancer tissues and cell lines. It functions as an oncogenic circRNA that promotes proliferation, migration and invasion while suppressing apoptosis, mainly through sponging miR-142-3p and increasing XIAP expression. Its high expression is associated with unfavorable clinicopathological characteristics, including enlarged tumor volume and advanced TNM stage. |
| Confidence score: |
0.7295 |