circRNA basic information
circBase ID: -
Name: hsa_circ_NRIP1
Synonym: Circular RNA Nuclear Receptor Interaction Protein 1
Host Gene: NRIP1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

xenograft tumors; CRC tumor tissues

Cell lines:

HCT116; SW480; NCM460; 293 T

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: SMURF1, p-AKT, Bcl-2, Bax
Associated microRNA: miR-195-5p
Biological function: circ-NRIP1 promotes CRC cell malignant behavior, including clonogenicity, migration and invasion, reduces 5-FU sensitivity, and inhibits apoptosis through AKT pathway activation.
Molecular mechanism: circ-NRIP1 competitively binds miR-195-5p, relieves miR-195-5p-mediated suppression of SMURF1, activates the AKT pathway, inhibits apoptosis, and decreases 5-FU sensitivity; curcumin enhances 5-FU sensitivity by downregulating circ-NRIP1.
Biological pathway or process:

PI3K/AKT (promotes); apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); chemoresistance (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model

Clinical significance:

The circ-NRIP1/miR-195-5p/SMURF1/AKT axis may provide a novel strategic approach for clinical CRC treatment by modulating 5-FU sensitivity.

Description:

In CRC cells, circ-NRIP1 is up-regulated and functions as a pro-malignant circRNA that promotes clonogenicity, migration and invasion while reducing sensitivity to 5-FU. Curcumin downregulates circ-NRIP1, thereby strengthening miR-195-5p-mediated inhibition of SMURF1, suppressing AKT pathway activation, promoting apoptosis, and enhancing 5-FU sensitivity.

Confidence score:

0.6542

Other information
Title:

Curcumin Modulates 5-fluorouracil Sensitivity in Colorectal Cancer Cells Through the circ-NRIP1/miR-195-5p/SMURF1/AKT Signaling Axis.

Journal: Journal of biochemical and molecular toxicology
Published: 2026
PubMed ID: 41796442
Study type:

biological research

Data availability: The data that supported the findings of this study are available on reasonable request from the corresponding author; Supporting Table 1: Primer sequence for qRT-PCR
Code availability: -