circRNA basic information
circBase ID: hsa_circ_0000497
Name: hsa_circ_SLAIN1
Synonym: circSLAIN1
Host Gene: SLAIN1
Genomic location(hg19): chr13:78293666-78327493:+
Genomic location(hg38): chr13:77719531-77753358:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: Glioblastoma
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioblastoma tumor tissues; adjacent non-tumor tissues; paracancerous tissues; xenograft tumors
Cell lines:

U87; U251; T98G; T98; LN229; HEB; THP-1; GL261; 293T
In vivo animal model:

cell line-derived xenograft; syngeneic model
circRNA-disease information
Expression pattern:
DN
Associated gene: NLRX1
Associated microRNA: miR-1225-3p
Biological function: circSLAIN1 acts as a tumor suppressor in GBM; it attenuates GBM cell proliferation, migration and invasion, enhances apoptosis, and suppresses M2 polarization of tumor-associated macrophages.
Molecular mechanism: circSLAIN1 functions as a cytoplasmic ceRNA that sponges miR-1225-3p, thereby relieving repression of NLRX1 and upregulating NLRX1 to inhibit GBM malignancy and M2-like TAM polarization.
Biological pathway or process:

ceRNA regulation (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); cell cycle (inhibits); EMT (inhibits); macrophage polarization (inhibits); immune regulation (promotes)
Detected method:
Q
H
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Microarray; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; ELISA; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circSLAIN1 expression is associated with longer overall survival in GBM patients and may serve as a favorable prognostic biomarker and therapeutic target.
Description:

circSLAIN1 (hsa_circ_0000497) is down-regulated in GBM and higher expression is associated with better overall survival. Functionally, circSLAIN1 suppresses GBM cell proliferation, migration, invasion, EMT-related features and M2-like TAM polarization while promoting apoptosis. Mechanistically, it acts as a cytoplasmic ceRNA sponge for miR-1225-3p to upregulate NLRX1, suggesting prognostic biomarker and therapeutic target potential.
Confidence score:

0.8991
Other information
Title:

CircRNA SLAIN1 is associated with reduced malignancy progression of glioblastoma and the M2-like polarization of tumor-associated macrophages via miR-1225-3p/NLRX1 axis.

Journal: International journal of biological macromolecules
Published: 2026
PubMed ID: 41794238
Study type:

combined biological and clinical study
Data availability: Data will be made available on request; Supplementary Table S2; Supplementary Table S3; Supplementary data: https://doi.org/10.1016/j.ijbiomac.2026.151212
Code availability: https://github.com/OpenGene/fastp; https://ccb.jhu.edu/software/hisat2; https://ccb.jhu.edu/software/stringtie; https://github.com/gpertea/gffcompare/; https://bioconductor.org/packages/release/bioc/html/edgeR.html