| Expression pattern: |
DN |
| Associated gene: |
ERBB2, AKT, p-AKT |
| Associated microRNA: |
- |
| Biological function: |
CircZBTB46 protects LPS-injured endothelial cells by increasing viability, reducing apoptosis, suppressing pro-inflammatory cytokines, decreasing ROS and MDA, and enhancing antioxidant enzymes SOD and CAT. |
| Molecular mechanism: |
CircZBTB46 modulates ERBB2-AKT signaling; its overexpression up-regulates ERBB2 expression and promotes AKT phosphorylation, and ERBB2 inhibition attenuates its protective effects. |
| Biological pathway or process: |
PI3K/AKT (promotes); apoptosis (inhibits); proliferation (promotes); inflammation (inhibits); other pathway/process (inhibits); oxidative phosphorylation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; ELISA; RNA-seq; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for sepsis-related acute kidney injury. |
| Description: |
In an LPS-induced HUVEC model of sepsis-associated acute kidney injury, circZBTB46 was down-regulated and its overexpression protected endothelial cells from injury. It increased cell viability, reduced apoptosis, inflammatory cytokine release and oxidative stress, and acted through ERBB2-AKT pathway activation; ERBB2 inhibition weakened these protective effects. |
| Confidence score: |
0.5089 |