circRNA basic information
circBase ID: -
Name: hsa_circ_PRKDC
Synonym: Circ‐PRKDC
Host Gene: PRKDC
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004963
MONDO name: T-cell acute lymphoblastic leukemia
Disease details: T-cell acute lymphoblastic leukemia
Disease DO ID:
 5603
Disease MeSH ID:
 -
Disease NCIt ID:
 C3183
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow samples
Cell lines:

Jurkat; TALL‐1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: RELN, PI3K, AKT, mTOR, Ago2
Associated microRNA: miR‐653‐5p
Biological function: Promotes proliferation and PI3K/AKT/mTOR activation; inhibits autophagy and apoptosis in T-ALL cells.
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-653-5p, thereby modulating RELN and PI3K/AKT/mTOR signaling.
Biological pathway or process:

PI3K/AKT/mTOR (promotes); autophagy (inhibits); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; TUNEL; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis
Clinical significance:

-
Description:

Circ-PRKDC is up-regulated in T-ALL bone marrow samples and T-ALL cell lines. Functionally, Circ-PRKDC promotes proliferation and PI3K/AKT/mTOR pathway activation while suppressing autophagy and apoptosis by sponging miR-653-5p to upregulate RELN.
Confidence score:

0.7032
Other information
Title:

The depletion of Circ-PRKDC enhances autophagy and apoptosis in T-cell acute lymphoblastic leukemia via microRNA-653-5p/Reelin mediation of the PI3K/AKT/mTOR signaling pathway.

Journal: The Kaohsiung journal of medical sciences
Published: 2021
PubMed ID: 33615686
Study type:

combined biological and clinical study
Data availability: -
Code availability: -