| Expression pattern: |
UP |
| Associated gene: |
p-Akt, c-Myc, Ki67, E-cadherin, N-cadherin |
| Associated microRNA: |
miR-1294 |
| Biological function: |
circ_0023984 promotes ESCC cell proliferation, migration, invasion, metastasis and cell cycle progression, and acts as an oncogenic circRNA in ESCC progression. |
| Molecular mechanism: |
circ_0023984 functions as a molecular sponge for miR-1294, represses miR-1294 expression, and activates the PI3K/Akt/c-Myc pathway by increasing p-Akt and c-Myc expression. |
| Biological pathway or process: |
PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0023984 overexpression was correlated with advanced clinical stage, larger tumor diameter and lymph node metastasis of ESCC patients. |
| Description: |
circ_0023984 is up-regulated in ESCC tissues and cell lines and is associated with larger tumor diameter and lymph node metastasis. Functionally, it promotes ESCC cell proliferation, migration, invasion, metastasis, and cell cycle progression. Mechanistically, circ_0023984 sponges miR-1294 and activates the PI3K/Akt/c-Myc pathway, increasing p-Akt and c-Myc expression. |
| Confidence score: |
0.7416 |