circRNA basic information
circBase ID: -
Name: hsa_circ_NFIX
Synonym: circNFIX
Host Gene: NFIX
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008170
MONDO name: ovarian cancer
Disease details: ovarian cancer
Disease DO ID:
 2394
Disease MeSH ID:
 D010051
Disease NCIt ID:
 C7431
Disease ICD11 ID:
 685124533
Disease OMIM ID:
 167000
Species: Human
Species details: Homo sapiens
Tissue specimen:

adjacent normal tissues; carcinoma tissues; ovarian tumor tissues; tumor tissues; lung tissues
Cell lines:

HOSEPiCs; PEO1; 3AO; SKOV3; OVCAR3; CAOV3; A2780; CD8 + T cells
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IL-6R, PD-L1, IGF2BP1, IGF2BP2, IGF2BP3, JAK1, STAT3, Ago2
Associated microRNA: miR-647
Biological function: circNFIX promotes ovarian cancer cell proliferation, viability, migration, invasion, EMT, metastasis, tumor growth and immune escape, while inhibiting apoptosis and CD8 + T cell antitumor activity.
Molecular mechanism: m6A-modified circNFIX is recognized by IGF2BP1/2/3, which maintains circNFIX expression; circNFIX functions as a ceRNA by sponging miR-647 to upregulate IL-6R, activate JAK1/STAT3 signaling, increase PD-L1 expression, and promote immune escape.
Biological pathway or process:

JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); immune regulation (promotes); ceRNA regulation (promotes); m6A modification (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; MeRIP / MeRIP-seq; Survival Analysis
Clinical significance:

circNFIX is overexpressed in OC and correlated with metastatic disease, advanced stage and poor prognosis; it may be a promising target for diagnosis, treatment and intervention of OC.
Description:

circNFIX is upregulated in ovarian cancer tissues and cell lines, and high circNFIX expression is associated with metastasis, advanced stage and poor prognosis. Mechanistically, m6A-modified circNFIX is maintained by IGF2BP1/2/3 recognition and promotes OC progression and immune escape by sponging miR-647 to upregulate IL-6R, activate JAK1/STAT3 signaling and increase PD-L1 expression.
Confidence score:

0.8148
Other information
Title:

m6A-modified circNFIX promotes ovarian cancer progression and immune escape via activating IL-6R/JAK1/STAT3 signaling by sponging miR-647.

Journal: International immunopharmacology
Published: 2023
PubMed ID: 37713785
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this published article; Supplementary data to this article can be found online at https://doi. org/10.1016/j.intimp.2023.110879.
Code availability: -