| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-671-5p |
| Biological function: |
circRNA-PTPRA knockdown protects against ox-LDL-induced HUVEC injury by increasing cell viability, reducing apoptosis, and suppressing inflammatory cytokine secretion. |
| Molecular mechanism: |
circRNA-PTPRA functions as a sponge for miR-671-5p; circRNA-PTPRA downregulation upregulates miR-671-5p to repress ox-LDL-induced endothelial cell injury. |
| Biological pathway or process: |
ceRNA regulation (promotes); apoptosis (promotes); inflammation (promotes); proliferation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot |
| Clinical significance: |
circRNA-PTPRA is upregulated in the serum of patients with AS and may provide early screening molecules and potential therapeutic targets for AS. |
| Description: |
circRNA-PTPRA is upregulated in the serum of AS patients and in ox-LDL-treated HUVECs. It promotes ox-LDL-induced endothelial cell injury by sponging miR-671-5p, while circRNA-PTPRA knockdown restores miR-671-5p, improves cell viability, reduces apoptosis, and suppresses inflammatory cytokine secretion. |
| Confidence score: |
0.7032 |