circRNA basic information
circBase ID: hsa_circ_0034447
Name: hsa_circ_THBS1
Synonym: circTHBS1
Host Gene: THBS1
Genomic location(hg19): chr15:39876188-39876388:+
Genomic location(hg38): chr15:39583987-39584187:+
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tissues; adjacent normal tissues; tumor tissues; adjacent noncancerous tissues
Cell lines:

KYSE150; TE1; KYSE30; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: NAT10, Rab8a
Associated microRNA: -
Biological function: Promotes ESCC cell proliferation, colony formation, migration, autophagic flux, tumor growth and malignant progression.
Molecular mechanism: circTHBS1 interacts with and stabilizes NAT10 by preventing ubiquitin-mediated proteasomal degradation, increases NAT10-mediated ac4C modification, and enhances Rab8a translation to drive autophagy and ESCC progression.
Biological pathway or process:

autophagy (promotes); proliferation (promotes); migration (promotes); ubiquitination (inhibits); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Transfection; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circTHBS1 expression is associated with histological grade, pT status, lymph node status, poor overall survival, and may serve as a diagnostic/prognostic biomarker in ESCC.
Description:

circTHBS1 is up-regulated in ESCC and functions as an oncogenic circRNA associated with poor prognosis. It binds and stabilizes NAT10 by preventing ubiquitin-mediated proteasomal degradation, thereby enhancing ac4C-dependent Rab8a translation, promoting autophagy, proliferation, migration, and tumor progression.
Confidence score:

0.8707
Other information
Title:

CircTHBS1 stabilizes N-acetyltransferase 10 to promote N4-acetylcytidine-dependent Rab8a translation and autophagy in esophageal squamous cell carcinoma.

Journal: International journal of biological macromolecules
Published: 2026
PubMed ID: 41722799
Study type:

combined biological and clinical study
Data availability: GSE131969; CircRiC database (https://hanlab.uth.edu/cRic); Supplementary data: https://doi.org/10.1016/j.ijbiomac.2026.150934
Code availability: -