| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
No disease-driving biological function was tested; circ_0051240 was used as an ovarian cancer-specific circRNA target for ultrasensitive detection and relative expression analysis in human cell lines. |
| Molecular mechanism: |
The study developed a toehold probe-mediated HCR/CRISPR-Cas12a fluorescence detection mechanism targeting the back-splice junction of circ_0051240; no endogenous disease mechanism was investigated. |
| Biological pathway or process: |
not specified |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Bioinformatics Analysis |
| Clinical significance: |
The assay has potential for accurate, highly sensitive quantification of ovarian cancer-specific circ_0051240 and for exploring early-stage diagnostic methods for ovarian cancer. |
| Description: |
This study focused on circ_0051240 as an ovarian cancer-specific circRNA target rather than investigating its biological function. The authors developed a Fe3O4-Au@UIO-66-NH2 toehold probe-mediated HCR/CRISPR-Cas12a fluorescent sensor that recognizes the back-splice junction of circ_0051240 and validated relative expression trends in human cell lines with RT-qPCR. circ_0051240 showed higher signal in SK-OV-3 ovarian cancer cells than in IOSE80 cells, supporting its potential value for ovarian cancer-related detection. |
| Confidence score: |
0.329 |