| Expression pattern: |
UP |
| Associated gene: |
HMGA2 |
| Associated microRNA: |
miR-497-5p |
| Biological function: |
Circ-CCND1 promotes HCC cell proliferation, migration, and invasion; knockdown of circ-CCND1 suppresses these malignant phenotypes. |
| Molecular mechanism: |
Circ-CCND1 acts as a ceRNA/molecular sponge for miR-497-5p to positively regulate HMGA2 expression, thereby promoting malignant phenotypes of HCC cells. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot |
| Clinical significance: |
High circ-CCND1 expression was associated with relatively large tumor size and lymph node metastasis in HCC patients; circ-CCND1 is proposed as a promising cancer biomarker and therapeutic target for HCC. |
| Description: |
Circ-CCND1 is up-regulated in HCC tissues and cell lines and is associated with larger tumor size and lymph node metastasis. It mainly localizes in the cytoplasm and promotes HCC cell proliferation, migration, and invasion by sponging miR-497-5p and up-regulating HMGA2 through a ceRNA mechanism. |
| Confidence score: |
0.7404 |