| Expression pattern: |
UP |
| Associated gene: |
TREM1, NF-kappaB P65, IL-1beta |
| Associated microRNA: |
miR-3921 |
| Biological function: |
Inhibits intracellular survival of M.tb in macrophages, promotes THP-1 cell proliferation, and promotes proinflammatory IL-1beta production through TREM1/NF-kappaB P65 signaling. |
| Molecular mechanism: |
hsa_circ_0004771 acts as a miRNA sponge for hsa-miR-3921, increasing TREM1 expression and NF-kappaB P65 phosphorylation, thereby promoting IL-1beta production and suppressing intracellular M.tb survival. |
| Biological pathway or process: |
NF-kappaB (promotes); inflammation (promotes); proliferation (promotes); ceRNA regulation (promotes); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; ELISA; Western Blot |
| Clinical significance: |
Potential target for the development of novel measures against TB control. |
| Description: |
hsa_circ_0004771 is upregulated in M.tb-infected THP-1 macrophages and functions as a host defense circRNA. It sponges hsa-miR-3921 to increase TREM1 expression, enhances NF-kappaB P65 phosphorylation and IL-1beta production, and thereby suppresses intracellular survival of M.tb. |
| Confidence score: |
0.7519 |