circRNA basic information
circBase ID: hsa_circ_0067934
Name: hsa_circ_PRKCI
Synonym: circ-PRKCI
Host Gene: PRKCI
Genomic location(hg19): chr3:170013698-170015181:+
Genomic location(hg38): chr3:170295910-170297393:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma / RCC
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

RCC tissues; peritumor normal tissue; serum; serum exosomes
Cell lines:

786-O; ACHN; Caki-1; 769-P; HK-2
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CCND1, AGO2
Associated microRNA: miR-545-3p
Biological function: Promotes proliferation, migration, and invasion of RCC cells; inhibits apoptosis; exosomal transfer from highly malignant RCC cells enhances malignant phenotypes in less malignant RCC cells.
Molecular mechanism: Exosomal circ-PRKCI acts as an miRNA sponge binding miR-545-3p, thereby regulating CCND1 to promote RCC proliferation.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Clinical Sample Validation
Clinical significance:

circ-PRKCI is upregulated in RCC tissues and serum exosomes and may serve as a novel liquid biopsy biomarker.
Description:

circ-PRKCI (hsa_circ_0067934) is upregulated in RCC tissues and serum exosomes and can be transferred from highly malignant Caki-1 cells to less malignant RCC cells via exosomes. It mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-545-3p to increase CCND1, thereby promoting RCC proliferation; it also enhances migration/invasion and inhibits apoptosis in RCC cells in exosome/circ-PRKCI gain-of-function settings.
Confidence score:

0.7302
Other information
Title:

Tumor Cell-Derived Exosomal circ-PRKCI Promotes Proliferation of Renal Cell Carcinoma via Regulating miR-545-3p/CCND1 Axis.

Journal: Cancers
Published: 2022
PubMed ID: 36612120
Study type:

combined biological and clinical study
Data availability: All the original data of this study are available from the corresponding author upon request.
Code availability: -