| Expression pattern: |
UP |
| Associated gene: |
HSP90, NOTCH1 |
| Associated microRNA: |
- |
| Biological function: |
promotes stemness (CSC-like properties), proliferation, migration, and invasion in gastric cancer cells |
| Molecular mechanism: |
circSLC4A7 physically interacts with HSP90 and activates NOTCH1/Notch signaling to regulate gastric cancer stemness and progression |
| Biological pathway or process: |
Notch (promotes); stemness (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNA Pull-Down; RIP (RNA Immunoprecipitation); IF (Immunofluorescence); Western Blot; Transfection; Wound Healing Assay; Transwell Assay; Colony Formation Assay; CCK8; Flow Cytometry（Non-apoptosis/cycle）; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circSLC4A7 is up-regulated in gastric cancer stem-like spheroid cells and GC tissues/cell lines. It promotes CSC-like properties and malignant phenotypes (proliferation, migration, invasion) by physically binding HSP90 and activating NOTCH1/Notch signaling. |
| Confidence score: |
0.8416 |