circRNA basic information
circBase ID: hsa_circ_0001079
Name: hsa_circ_STK39
Synonym: circSTK39
Host Gene: STK39
Genomic location(hg19): chr2:169018296-169038600:-
Genomic location(hg38): chr2:168161786-168182090:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BCa tissues; matched adjacent nontumor bladder tissues; bladder tissues; tumor tissues; lung tumor tissues
Cell lines:

5637; T24; EJ; 253 J-BV; SV-HUC-1; HEK-293 T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: NR3C2, AGO2, STK39
Associated microRNA: miR-135a-5p
Biological function: Suppresses proliferation, colony formation, migration, invasion, tumor development, metastasis, and EMT of bladder cancer cells.
Molecular mechanism: circSTK39 acts as a miRNA sponge for miR-135a-5p, upregulates NR3C2, and inhibits NR3C2-mediated EMT signaling in BCa.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis
Clinical significance:

Lower circSTK39 expression was associated with worse prognosis, advanced pathology stage, shorter overall survival, and potential diagnostic value; circSTK39 is proposed as a promising biomarker for diagnosis and prognosis of BCa patients.
Description:

circSTK39/hsa_circ_0001079 is a human STK39-derived circRNA that is downregulated in bladder cancer tissues and cell lines. It acts as a tumor-suppressive circRNA by sponging miR-135a-5p, increasing NR3C2 expression, and inhibiting EMT-associated proliferation, migration, invasion, tumor growth, and metastasis. Higher circSTK39 expression is associated with longer overall survival, and the study proposes circSTK39 as a diagnostic and prognostic biomarker for BCa.
Confidence score:

0.8981
Other information
Title:

CircSTK39 suppresses the proliferation and invasion of bladder cancer by regulating the miR-135a-5p/NR3C2-mediated epithelial-mesenchymal transition signaling pathway.

Journal: Cell biology and toxicology
Published: 2023
PubMed ID: 36538242
Study type:

combined biological and clinical study
Data availability: published article and its supplementary information files; GSE97239
Code availability: -