| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, PTGS2 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Regulates PCOS-related granulosa cell dysfunction and lipid metabolism; overexpression reduces cell viability and proliferation, decreases OCR, and increases apoptosis, oxidative stress, lipid accumulation, TC, TG, and lipid droplet formation. |
| Molecular mechanism: |
circ_0075691 interacts with the RNA-binding protein EIF4A3, enhances EIF4A3 protein stability, and promotes EIF4A3-mediated PTGS2 mRNA stability. |
| Biological pathway or process: |
lipid metabolism (promotes); apoptosis (promotes); proliferation (inhibits); mitochondrial function (inhibits); mRNA stability (promotes); oxidative phosphorylation (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; TUNEL; Western Blot; In Vivo Animal Model; H&E Staining; ELISA; Bioinformatics Analysis |
| Clinical significance: |
circ_0075691 is elevated in follicular fluid and granulosa cells from PCOS patients and the circ_0075691/EIF4A3/PTGS2 axis may provide potential therapeutic targets for PCOS. |
| Description: |
circ_0075691 is a human ATXN1-derived circRNA that is up-regulated in follicular fluid and granulosa cells from PCOS patients. It promotes PCOS-associated granulosa cell lipid metabolic dysfunction, oxidative stress, apoptosis, and reduced energy metabolism by interacting with EIF4A3, stabilizing EIF4A3 protein, and enhancing PTGS2 mRNA stability. The circ_0075691/EIF4A3/PTGS2 axis is proposed as a mechanistic contributor and potential therapeutic target in PCOS. |
| Confidence score: |
0.8279 |