| Expression pattern: |
DN |
| Associated gene: |
arachidonic acid, AA, phospholipase, PLA2, EIF4A3, U2AF65, 56 aa protein |
| Associated microRNA: |
- |
| Biological function: |
Inhibits breast cancer cell proliferation and invasion in vitro; inhibits tumor growth and metastasis in vivo; acts as a tumor suppressor; down-regulates arachidonic acid and decreases AA metabolites. |
| Molecular mechanism: |
circNFIB encodes a 56 aa protein and may inhibit synthesis of arachidonic acid by regulating phospholipase; EIF4A3 and U2AF65 may regulate circNFIB expression by binding to its flanking sequence. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); metastasis (inhibits); lipid metabolism (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Transfection; MTT; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); ELISA; Western Blot; Bioinformatics Analysis; RNA-seq |
| Clinical significance: |
CircNFIB and its encoded protein may serve as therapy targets and biomarkers for breast cancer in the future. |
| Description: |
circNFIB (hsa_circ_0086376), derived from NFIB, is down-regulated in breast cancer tissues and cells and functions as a tumor suppressor. It inhibits breast cancer proliferation, invasion, tumor growth, and metastasis, potentially through protein-coding capacity and suppression of arachidonic acid synthesis via phospholipase regulation. EIF4A3 and U2AF65 are proposed upstream regulators of circNFIB expression. |
| Confidence score: |
0.8109 |