| Expression pattern: |
UP |
| Associated gene: |
TLR4, MyD88, NF-kB p65, IL-6, AGO2, Ki-67 |
| Associated microRNA: |
miR-326 |
| Biological function: |
Promotes proliferation, migration, invasion, carcinogenesis and tumor growth of CCA cells; contributes to LPS-induced anti-apoptosis of CCA cells. |
| Molecular mechanism: |
circ_0000591 acts as a ceRNA sponge for miR-326, thereby regulating TLR4 and activating the TLR4/MyD88/NF-kB/IL-6 pathway to promote CCA progression and LPS-induced anti-apoptosis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); NF-kappaB (promotes); inflammation (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0000591 is up-regulated in cholangiocarcinoma tissues and cell lines. It promotes CCA cell proliferation, migration, invasion and in vivo tumorigenesis by sponging miR-326 and activating the TLR4/MyD88/NF-kB/IL-6 axis. Silencing circ_0000591 suppresses tumor growth and attenuates LPS-induced anti-apoptosis. |
| Confidence score: |
0.8275 |