circRNA basic information
circBase ID: hsa_circ_0007444
Name: hsa_circ_RHOBTB3
Synonym: circRHOBTB3
Host Gene: RHOBTB3
Genomic location(hg19): chr5:95091099-95099324:+
Genomic location(hg38): chr5:95755395-95763620:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa tissue; adjacent non-cancerous prostate tissue; metastatic PCa tissue; non-metastatic tissue
Cell lines:

22RV1; DU145; PC-3; RWPE-1; HEK-293T; HLEC; HUVEC
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: NONO, MAOA, SRSF9, AluSx, AluJb
Associated microRNA: -
Biological function: circRHOBTB3 inhibits PCa cell proliferation, viability, colony formation, migration, invasion, and metastasis; it acts as a tumor suppressor in PCa.
Molecular mechanism: circRHOBTB3 binds NONO and sequesters it in the cytoplasm, preventing NONO from acting as a transcription factor to promote MAOA transcription; SRSF9 binds AluSx and AluJb to inhibit circRHOBTB3 circularization.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); other pathway/process (other)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

Low expression of circRHOBTB3 correlates with elevated pathological T stage, clinical M stage, and D'Amico grade; circRHOBTB3 may be a promising biomarker for mPCa detection and intervention.
Description:

circRHOBTB3 is a human RHOBTB3-derived circRNA that is downregulated in prostate cancer and more strongly reduced in metastatic PCa. It suppresses PCa proliferation, migration, invasion, and metastasis by binding NONO, retaining NONO in the cytoplasm, and thereby reducing NONO-mediated MAOA transcription. Clinically, low circRHOBTB3 expression is associated with higher pathological T stage, clinical M stage, and D'Amico grade, suggesting biomarker potential for metastatic PCa.
Confidence score:

0.8601
Other information
Title:

CircRHOBTB3 suppresses MAOA by promoting cytoplasmic retention of NONO to inhibit prostate cancer proliferation and metastasis.

Journal: Cancer letters
Published: 2025
PubMed ID: 40680813
Study type:

combined biological and clinical study
Data availability: available from the corresponding author upon request; Supplementary data: https://doi.org/10.1016/j.canlet.2025.217910
Code availability: -