| Expression pattern: |
UP |
| Associated gene: |
SRSF1, KLF12, IL-8 |
| Associated microRNA: |
- |
| Biological function: |
promotes NSCLC cell viability, migration and invasion; suppresses apoptosis; promotes tumor growth in vivo; promotes macrophage M2 polarization |
| Molecular mechanism: |
circ_0007432 binds the RBP SRSF1 to stabilize KLF12 mRNA; KLF12 transcriptionally activates IL-8 by binding the IL-8 promoter, increasing IL-8 release and promoting malignant behaviors and M2 macrophage polarization |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); macrophage polarization (promotes); immune regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; ELISA; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
higher expression of circ_0007432 contributed to poor prognosis of NSCLC patients |
| Description: |
circ_0007432 is up-regulated in NSCLC tissues/cells and mainly localized in the cytoplasm. It promotes NSCLC malignant phenotypes and tumor growth and drives macrophage M2 polarization by binding SRSF1 to stabilize KLF12 mRNA, which in turn transcriptionally activates IL-8 and increases IL-8 release. High circ_0007432 expression is associated with poor patient prognosis. |
| Confidence score: |
0.8831 |