circRNA basic information
circBase ID: hsa_circ_0093887
Name: hsa_circ_SIRT1
Synonym: circSirt1
Host Gene: SIRT1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005311
MONDO name: atherosclerosis
Disease details: atherosclerosis
Disease DO ID:
 1936
Disease MeSH ID:
 D050197
Disease NCIt ID:
 C35768
Disease ICD11 ID:
 109367356
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

vascular smooth muscle cells; vessels surrounding the atherosclerotic plaque; healthy vessels adjacent to the atherosclerotic plaque
Cell lines:

AoSMCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: GLS / glutaminase
Associated microRNA: miR-27b-3p
Biological function: circSirt1 protects vascular smooth muscle cells against oxidative stress-induced injury, cell death and apoptosis during atherosclerosis, and promotes glutamine metabolism.
Molecular mechanism: circSirt1 functions as a miR-27b-3p sponge to relieve miR-27b-3p-mediated suppression of GLS, thereby restoring glutamine metabolism and protecting VSMCs from oxidative stress-induced injury.
Biological pathway or process:

apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes); other pathway/process (inhibits)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot
Clinical significance:

circSirt1 may be a therapeutic target or approach for atherosclerosis treatment.
Description:

circSirt1 is down-regulated in VSMCs from atherosclerosis patients and under oxidative stress. Its overexpression protects AoSMCs/VSMCs from oxidative stress-induced injury and apoptosis by sponging miR-27b-3p, increasing GLS expression, and restoring glutamine metabolism. The circSirt1-miR-27b-3p-GLS axis is proposed as a potential therapeutic mechanism for maintaining VSMC homeostasis in atherosclerosis.
Confidence score:

0.6996
Other information
Title:

Circular RNA-Sirt1 sponges miR-27b-3p to protect vascular smooth muscle cell injury during atherosclerosis through regulating the glutamine metabolism pathway.

Journal: Cytotechnology
Published: 2025
PubMed ID: 40521053
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request; supplementary material available at https:// doi. org/ 10. 1007/ s10616-025-00759-x
Code availability: -