| Expression pattern: |
UP |
| Associated gene: |
E2F4, FAM64A, RelA, GLI4, IL-6, CCND1, CDC2, CCNB1, p65 |
| Associated microRNA: |
miR-541-3p |
| Biological function: |
Promotes breast cancer cell proliferation and cell cycle progression, inhibits apoptosis, and accelerates tumor growth in vivo. |
| Molecular mechanism: |
E2F4 transcriptionally activates circ_0041732 from FAM64A; circ_0041732 acts as a ceRNA sponge for miR-541-3p to up-regulate RelA and GLI4, activating NF-kB and Hedgehog pathways. |
| Biological pathway or process: |
NF-kappaB (promotes); Hedgehog (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0041732 is an oncogenic circRNA up-regulated in breast cancer cells and tissues. Its up-regulation is driven by E2F4-mediated transcriptional activation of its host gene FAM64A, and it promotes proliferation, cell cycle progression, and xenograft tumor growth while suppressing apoptosis by sponging miR-541-3p to increase RelA and GLI4 and activate NF-kB and Hedgehog signaling. |
| Confidence score: |
0.8478 |