| Expression pattern: |
UP |
| Associated gene: |
ARG1, Pg3G |
| Associated microRNA: |
miR-4521 |
| Biological function: |
circHMGCS1 has a propathological role in HFHG-induced vascular endothelial dysfunction by disrupting endothelial homeostasis, reducing nitric oxide bioavailability and eNOS activity, increasing ROS, and promoting inflammatory adhesion molecule expression; circHMGCS1 knockdown reproduces protective effects, whereas overexpression counteracts Pg3G-mediated endothelial protection. |
| Molecular mechanism: |
circHMGCS1 functions through a ceRNA mechanism by sponging miR-4521 to regulate ARG1 expression, thereby affecting nitric oxide bioavailability and oxidative stress; Pg3G binds circHMGCS1 and acts as a functional antagonist. |
| Biological pathway or process: |
ceRNA regulation (promotes); inflammation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Transfection; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circHMGCS1 is investigated as a pathogenic circRNA in T2DM-associated vascular endothelial dysfunction. Pg3G suppresses and directly interacts with circHMGCS1, restoring the miR-4521/ARG1 axis and improving NO bioavailability, eNOS activity, oxidative stress, and inflammatory adhesion molecule expression. circHMGCS1 knockdown mimics Pg3G protection, while circHMGCS1 overexpression counteracts it. |
| Confidence score: |
0.4376 |