| Expression pattern: |
UP |
| Associated gene: |
HOXA10, LIF, rhG-CSF |
| Associated microRNA: |
- |
| Biological function: |
hsa circ 0001550 acts as a negative regulator of embryo adhesion and embryo implantation; overexpression reduces adhesion of BeWo or JAR spheroids and murine blastocysts to Ishikawa cells and reduces implantation sites in vivo, whereas knockdown improves embryo adhesion and upregulates HOXA10 and LIF. |
| Molecular mechanism: |
rhG-CSF downregulates hsa circ 0001550, relieving its suppression of endometrial receptivity markers HOXA10 and LIF; hsa circ 0001550 overexpression suppresses embryo adhesion and implantation, while knockdown increases HOXA10 and LIF mRNA expression and improves adhesion. |
| Biological pathway or process: |
other pathway/process (inhibits); proliferation (promotes); cell cycle (other); apoptosis (other); angiogenesis (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Transfection; CCK8; EdU Staining; In Vivo Animal Model; TUNEL; H&E Staining; IF (Immunofluorescence) |
| Clinical significance: |
hsa circ 0001550 is highly expressed in secretions and upregulated in the mid-secretory endometrium of patients with RIF, and is highlighted as a potential diagnostic biomarker and therapeutic target for infertility. |
| Description: |
This study identifies hsa circ 0001550 as a human circRNA derived from RARS and a negative regulator of endometrial receptivity, embryo adhesion, and embryo implantation. It is reported as upregulated in RIF-related samples, and functional overexpression/knockdown experiments show that it suppresses adhesion and implantation partly through downregulation of HOXA10 and LIF. rhG-CSF improves implantation partly by downregulating hsa circ 0001550, suggesting potential biomarker and therapeutic relevance in infertility and implantation failure. |
| Confidence score: |
0.4732 |