| Expression pattern: |
UP |
| Associated gene: |
PD-L1, METTL3, Ago2 |
| Associated microRNA: |
miR-3139 |
| Biological function: |
Promotes TNBC cell proliferation, invasion, immune escape, and tumor growth; silencing reduces tumor burden and enhances CD8+ T cell-associated antitumor immune activity. |
| Molecular mechanism: |
METTL3-mediated m6A methylation stabilizes or upregulates circ_0006174; circ_0006174 acts as a ceRNA sponge for miR-3139, leading to upregulation of PD-L1 and promotion of TNBC progression and immune evasion. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (promotes); m6A modification (other); apoptosis (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; MeRIP / MeRIP-seq; Transfection; CCK8; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; Nuclear-Cytoplasmic Fractionation; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); TUNEL; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Overexpression of circ_0006174 is associated with poor prognosis, shorter overall survival, increased lymph node metastasis, and higher TNM stage in TNBC patients; it is proposed as a potential therapeutic target. |
| Description: |
circ_0006174 is upregulated in TNBC tissues and cell lines and high expression is linked to poor prognosis. METTL3-mediated m6A methylation regulates circ_0006174, which sponges miR-3139 to increase PD-L1 expression, thereby promoting TNBC proliferation, invasion, tumor growth, and immune escape. |
| Confidence score: |
0.8868 |