circRNA basic information
circBase ID: -
Name: hsa_circ_TP63
Synonym: circTP63-N
Host Gene: TP63
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC biopsy tissue samples; nasopharyngeal epithelial tissue samples; subcutaneous tumor tissues; lung metastatic lesions
Cell lines:

CNE2; HONE1; 5-8F; HNE2; C666-1; NP69
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: HSP90AB1, LATS1/2, YAP1, INHBA, MMP3, CCNE2
Associated microRNA: -
Biological function: circTP63-N functions as a tumor suppressor in NPC and inhibits NPC cell proliferation, invasion, migration, and metastasis.
Molecular mechanism: circTP63-N binds HSP90AB1, facilitates recruitment of LATS1/2 and YAP1, promotes YAP1 phosphorylation and ubiquitination-mediated degradation, reduces YAP1 nuclear translocation and transcriptional activity, and suppresses downstream oncogenic genes INHBA, MMP3, and CCNE2 through the Hippo pathway.
Biological pathway or process:

Hippo/YAP (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); cell cycle (inhibits); ubiquitination (promotes); other pathway/process (inhibits)
Detected method:
Q
H
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; FISH / smFISH; ISH (In Situ Hybridization); IHC (Immunohistochemistry); IF (Immunofluorescence); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis
Clinical significance:

circTP63-N may serve as a novel potential molecular marker and therapeutic target for clinical diagnosis and treatment of NPC.
Description:

circTP63-N is a novel TP63-derived circRNA that is significantly downregulated in nasopharyngeal carcinoma. It acts as a tumor suppressor by binding HSP90AB1, recruiting LATS1/2 and YAP1, promoting YAP1 phosphorylation and ubiquitination-dependent degradation, and suppressing YAP1/Hippo downstream oncogenic programs including INHBA, MMP3, and CCNE2. Restoration of circTP63-N inhibits NPC proliferation, invasion, migration, and metastasis in vitro and in vivo.
Confidence score:

0.8869
Other information
Title:

circTP63-N suppresses the proliferation and metastasis of nasopharyngeal carcinoma via engaging with HSP90AB1 to modulate the YAP1/Hippo signaling pathway.

Journal: Science China. Life sciences
Published: 2025
PubMed ID: 39754006
Study type:

combined biological and clinical study
Data availability: PRJNA391554; GSE12452; GSE53819; https://doi.org/10.1007/s11427-023-2737-2
Code availability: -