circRNA basic information
circBase ID: hsa_circ_0001546
Name: hsa_circ_FAM114A2
Synonym: circ_0001546
Host Gene: FAM114A2
Genomic location(hg19): chr5:153413350-153414527:-
Genomic location(hg38): chr5:154033790-154034967:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer / GC
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tissues; corresponding adjacent normal stomach mucosa tissues; blood samples; plasma samples
Cell lines:

GES-1; MKN-45; HGC-27; HGC-27/L-OHP; AGS; SGC-7901; BGC-823; HEK 293T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: AGO2, ATM, Chk2, p53
Associated microRNA: miR-421
Biological function: Inhibits proliferation and chemoresistance of gastric cancer cells, increases L-OHP-induced apoptosis, and sensitizes HGC-27/L-OHP cells to L-OHP.
Molecular mechanism: circ_0001546 acts as a miR-421 sponge to upregulate ATM and activates the ATM/Chk2/p53-dependent signaling pathway.
Biological pathway or process:

proliferation (inhibits); apoptosis (promotes); chemoresistance (inhibits); drug resistance (inhibits); p53 signaling (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis; RT-ddPCR
Clinical significance:

Lower circ_0001546 expression indicates poor prognosis, and circ_0001546 might be a potential diagnostic/prognostic biomarker and therapeutic target for cisplatin-based therapy resistant GC patients.
Description:

hsa_circ_0001546 is downregulated in gastric cancer tissues, plasma before tumor removal, and gastric cancer cell lines, and lower expression is associated with shorter overall survival. It functions as a miR-421 sponge, increases ATM expression, activates the ATM/Chk2/p53 pathway, suppresses proliferation and chemoresistance, and enhances L-OHP-induced apoptosis.
Confidence score:

0.8461
Other information
Title:

Hsa_circ_0001546 acts as a miRNA-421 sponge to inhibit the chemoresistance of gastric cancer cells via ATM/Chk2/p53-dependent pathway.

Journal: Biochemical and biophysical research communications
Published: 2020
PubMed ID: 31668372
Study type:

combined biological and clinical study
Data availability: Transparency document related to this article can be found online at https://doi.org/10.1016/j.bbrc.2019.10.117
Code availability: -