circRNA basic information
circBase ID: hsa_circ_0094606
Name: hsa_circ_SORBS1
Synonym: circ_0094606
Host Gene: SORBS1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer / PCa
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

PCa specimens; paired non-tumor tissues; tumor tissues; xenografts
Cell lines:

DU145; PC3; NCI-H660; RWPE-1; THP-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PRMT1, ILF3, IL-8 mRNA
Associated microRNA: -
Biological function: Promotes PCa cell proliferation, migration, EMT progression, in vivo tumorigenesis, and macrophage M2 polarization.
Molecular mechanism: circ_0094606 binds PRMT1, promoting PRMT1-mediated arginine methylation of ILF3 at R609, stabilizing ILF3 protein; ILF3 stabilizes IL-8 mRNA, leading to increased IL-8 expression and macrophage M2 polarization.
Biological pathway or process:

proliferation (promotes); migration (promotes); EMT (promotes); metastasis (promotes); mRNA stability (promotes); immune regulation (promotes); macrophage polarization (promotes); other pathway/process (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Western Blot; Bioinformatics Analysis
Clinical significance:

circ_0094606 was highly expressed in 70% of PCa patients and was proposed as a potential novel effective target for PCa treatment.
Description:

hsa_circ_0094606/circ_0094606 is up-regulated in prostate cancer tissues and cells. It promotes PCa proliferation, migration, EMT, xenograft growth, and macrophage M2 polarization by binding PRMT1, enhancing PRMT1-mediated ILF3 arginine methylation, and increasing IL-8 mRNA stability/expression. The study proposes circ_0094606 as a potential therapeutic target for PCa.
Confidence score:

0.8856
Other information
Title:

Hsa_circ_0094606 promotes malignant progression of prostate cancer by inducing M2 polarization of macrophages through PRMT1-mediated arginine methylation of ILF3.

Journal: Carcinogenesis
Published: 2023
PubMed ID: 36394342
Study type:

combined biological and clinical study
Data availability: The data of this study are available from the corresponding author on reasonable request.
Code availability: -