| Expression pattern: |
UP |
| Associated gene: |
IRGQ |
| Associated microRNA: |
miR-1294 |
| Biological function: |
Circ_0000854 promoted HCC cell proliferation, cell cycle progression, migration, invasion, tumor growth and metastasis; knockdown of circ_0000854 suppressed these malignant behaviors. |
| Molecular mechanism: |
Circ_0000854 functions as a miR-1294 sponge to upregulate IRGQ expression, thereby accelerating HCC progression through the circ_0000854/miR-1294/IRGQ ceRNA axis. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Clinical Sample Validation; Survival Analysis; Nuclear-Cytoplasmic Fractionation; Transfection; MTT; EdU Staining; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0000854 expression was associated with significantly lower survival rate in HCC patients. |
| Description: |
This study identified hsa_circ_0000854/circZCCHC2 as an up-regulated oncogenic circRNA in hepatocellular carcinoma. Circ_0000854 mainly localizes in the cytoplasm and promotes proliferation, cell cycle progression, migration, invasion, EMT-related protein changes, tumor growth and metastasis by sponging miR-1294 to increase IRGQ expression. High circ_0000854 expression was associated with poorer survival in HCC patients. |
| Confidence score: |
0.7981 |