circRNA basic information
circBase ID: -
Name: hsa_circ_COL5A1
Synonym: circular RNA collagen Valpha1 chain
Host Gene: COL5A1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005086
MONDO name: renal cell carcinoma
Disease details: renal cell carcinoma
Disease DO ID:
 4450
Disease MeSH ID:
 D002292
Disease NCIt ID:
 -
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; normal para-cancerous tissue
Cell lines:

Caki-1; A498; 786-O; 769P; HK2
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: KPNA2, Ago2
Associated microRNA: miR-3940-3p
Biological function: Promotes RCC cell proliferation, migration, invasion, EMT and glycolysis, and inhibits apoptosis.
Molecular mechanism: CircCOL5A1 acts as a cytoplasmic miR-3940-3p sponge to up-regulate KPNA2, thereby promoting RCC progression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); glycolysis (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Western Blot; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; Survival Analysis; ROC Analysis
Clinical significance:

High circCOL5A1 expression is associated with poor overall survival, diagnostic value for distinguishing RCC patients from healthy people, and clinicopathological features including tumor size, lymph node metastasis, TNM stage and distant metastasis.
Description:

CircCOL5A1 is up-regulated in RCC tissues and cell lines and is associated with poor prognosis, diagnostic potential, and aggressive clinicopathological features. Mechanistically, cytoplasmic circCOL5A1 sponges miR-3940-3p to increase KPNA2 expression, thereby promoting RCC cell proliferation, migration, invasion, EMT and glycolysis while inhibiting apoptosis.
Confidence score:

0.8595
Other information
Title:

Circular RNA collagen type V alpha 1 exerts a carcinogenic role in renal cell carcinoma via modulating microRNA-3940-3p/Karyopherin alpha2 axis.

Journal: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
Published: 2025
PubMed ID: 41364173
Study type:

combined biological and clinical study
Data availability: The datasets used and analyzed during the present study are available from the corresponding author on reasonable request.
Code availability: -