| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation, migration, and invasion and inhibits apoptosis in MDA-MB-231 cells; mediates pirarubicin inhibiting the malignant phenotype of MDA-MB-231 cells; acts as a potential diagnostic and prognostic biomarker in plasma exosomes. |
| Molecular mechanism: |
Pirarubicin downregulates circSTIL expression; circSTIL overexpression partially reverses the inhibitory effect of pirarubicin on proliferation, migration, invasion, and apoptosis in MDA-MB-231 cells, but downstream signaling pathways and molecular interactions remain unclear. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Plasma exosomal circSTIL has potential diagnostic value for distinguishing TNBC from non-TNBC, and high expression is associated with poorer DFS but not OS. |
| Description: |
circSTIL is an up-regulated human circRNA in TNBC cell lines, TNBC tissues, and plasma exosomes. Functionally, circSTIL promotes proliferation, migration, and invasion while inhibiting apoptosis in MDA-MB-231 cells, and its overexpression partially reverses the anti-malignant effects of pirarubicin. Clinically, plasma exosomal circSTIL may help distinguish TNBC from non-TNBC and high expression is associated with poorer DFS. |
| Confidence score: |
0.8319 |