| Expression pattern: |
UP |
| Associated gene: |
NCAPH |
| Associated microRNA: |
miR-328-3p |
| Biological function: |
Promotes CRC cell migration, invasion, EMT, and tumor metastasis to the liver and lung. |
| Molecular mechanism: |
circEIF3I acts as a ceRNA sponge for miR-328-3p to upregulate NCAPH expression, thereby promoting CRC metastasis. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circEIF3I expression was significantly correlated with tumor size and metastasis of CRC patients; the circEIF3I/miR-328-3p/NCAPH axis represents a novel therapeutic target for CRC. |
| Description: |
circEIF3I is up-regulated in colorectal cancer tissues and cell lines and correlates with tumor size and metastasis. It promotes CRC migration, invasion, EMT, and liver/lung metastasis by sponging miR-328-3p to enhance NCAPH expression, suggesting the circEIF3I/miR-328-3p/NCAPH axis as a potential therapeutic target. |
| Confidence score: |
0.7785 |