| Expression pattern: |
UP |
| Associated gene: |
METTL3, YTHDC1, USP13, Ago2 |
| Associated microRNA: |
miR-378a-5p |
| Biological function: |
Promotes cell cycle progression, proliferation, invasion, migration, tumor growth and metastasis of gastric cancer cells. |
| Molecular mechanism: |
METTL3-mediated m6A modification of circCDYL at sites 471 and 500 is recognized by YTHDC1, promoting nuclear export; cytoplasmic circCDYL acts as a miR-378a-5p sponge to regulate USP13 expression and promote gastric cancer progression. |
| Biological pathway or process: |
m6A modification (other); ceRNA regulation (promotes); cell cycle (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; MeRIP / MeRIP-seq; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircCDYL is expected to be a potential diagnostic biomarker and therapeutic target for gastric cancer. |
| Description: |
This study identifies human circCDYL (hsa_circ_0008285) as an up-regulated oncogenic circRNA in gastric cancer. METTL3-mediated m6A modification enables YTHDC1-dependent nuclear export of circCDYL, and cytoplasmic circCDYL promotes GC progression by sponging miR-378a-5p to increase USP13 expression. CircCDYL is proposed as a potential diagnostic biomarker and therapeutic target for gastric cancer. |
| Confidence score: |
0.8536 |