| Expression pattern: |
UP |
| Associated gene: |
PTBP1, GRN pre-mRNA, PGRN, NF-kappaB |
| Associated microRNA: |
- |
| Biological function: |
Promotes EMT and extracellular matrix (ECM) remodeling, leading to airway remodeling; inhibits anoikis/apoptosis; exacerbates emphysema in COPD models. |
| Molecular mechanism: |
circFCHO2 binds PTBP1 and promotes its nuclear import, increasing PTBP1 binding to GRN pre-mRNA to inhibit splicing, reducing GRN mRNA and PGRN protein/secretion, thereby activating NF-kappaB signaling to drive EMT and ECM remodeling. |
| Biological pathway or process: |
NF-kappaB (promotes); EMT (promotes); invasion (other); migration (other); apoptosis (inhibits); ceRNA regulation (inhibits); other pathway/process (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; TUNEL; IF (Immunofluorescence); IHC (Immunohistochemistry); ELISA; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
circFCHO2 is upregulated in lung tissue of COPD patients and may be clinically important for COPD pathogenesis. |
| Description: |
circFCHO2 is upregulated in COPD and promotes airway remodeling by driving EMT and ECM remodeling. It binds the splicing factor PTBP1 to enhance PTBP1 nuclear translocation, repressing GRN pre-mRNA splicing and lowering PGRN, which activates NF-kappaB signaling; circFCHO2 knockdown ameliorates emphysema/airway remodeling in cigarette-smoke COPD mice. |
| Confidence score: |
0.868 |