| Expression pattern: |
DN |
| Associated gene: |
IGF2BP3, AKT, mTOR |
| Associated microRNA: |
- |
| Biological function: |
circCGNL1 acts as a tumor-suppressive circRNA in NPC; its overexpression inhibits NPC cell proliferation, migration, invasion, and tumor growth while promoting apoptosis. |
| Molecular mechanism: |
circCGNL1 directly binds the RNA-binding protein IGF2BP3 and suppresses AKT/mTOR signaling by reducing AKT and mTOR phosphorylation. |
| Biological pathway or process: |
PI3K/AKT/mTOR (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; TUNEL; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circCGNL1 may serve as a promising diagnostic biomarker and therapeutic target for nasopharyngeal carcinoma. |
| Description: |
circCGNL1 is downregulated in nasopharyngeal carcinoma and functions as a tumor-suppressive circRNA. It directly interacts with the RNA-binding protein IGF2BP3, reduces phosphorylation of AKT and mTOR, inhibits AKT/mTOR signaling, and thereby suppresses NPC proliferation, migration, invasion, and tumor growth while promoting apoptosis. The study suggests circCGNL1 as a potential diagnostic biomarker and therapeutic target for NPC. |
| Confidence score: |
0.8625 |