| Expression pattern: |
UP |
| Associated gene: |
GOT1 |
| Associated microRNA: |
miR-433-3p |
| Biological function: |
Promotes proliferation, migration, invasion and glutamine catabolism, and inhibits apoptosis in pancreatic cancer; its knockdown represses tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-433-3p to upregulate GOT1, thereby promoting pancreatic cancer progression and glutamine catabolism. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ-MBOAT2 may be a therapeutic target for pancreatic cancer; circ-MBOAT2 expression was higher in stage III-IV pancreatic cancer tissues than in stage I-II. |
| Description: |
circ-MBOAT2 is up-regulated in pancreatic cancer tissues and cell lines and mainly localizes to the cytoplasm. Functionally, circ-MBOAT2 promotes malignant phenotypes and glutamine catabolism by sponging miR-433-3p to derepress GOT1, and its knockdown suppresses tumor growth in vivo. |
| Confidence score: |
0.7421 |