circRNA basic information
circBase ID: -
Name: hsa_circ_CCDC66
Synonym: -
Host Gene: CCDC66
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue; adjacent non-tumor tissues; colon tissue; polyp tissues
Cell lines:

HCT116; HT-29; Caco-2; LS123; CCD 841 CoN; 3T3L1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MYC, DNMT3B, EZH2, YAP1
Associated microRNA: miR-33b, miR-93, miR-185, miR-670, miR-4326
Biological function: promotes cell proliferation; promotes migration; promotes invasion; promotes anchorage-independent growth; promotes metastasis; promotes tumor growth in vivo
Molecular mechanism: Acts as a miRNA sponge (ceRNA-like) to protect oncogene transcripts (e.g., MYC) from miRNA-mediated repression, thereby increasing oncogene expression.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; BrdU; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); ROC Analysis; Survival Analysis
Clinical significance:

Elevated circCCDC66 in polyps and colon cancer; high circCCDC66 is associated with poor prognosis (lower overall survival) and shows good predictive performance in ROC analysis for CRC detection/prognosis.
Description:

circCCDC66 (circCCDC66_10_8) is up-regulated in colorectal/colon cancer tissues (including polyps) and its high expression predicts poorer overall survival. Functionally, it promotes proliferation, migration, invasion, anchorage-independent growth and metastasis, and its knockdown suppresses tumor growth and liver nodule formation in xenograft/orthotopic models. Mechanistically, it acts as a miRNA sponge (e.g., for miR-33b and miR-93), protecting oncogene mRNAs such as MYC and increasing oncogenic programs.
Confidence score:

0.8824
Other information
Title:

Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis.

Journal: Cancer research
Published: 2017
PubMed ID: 28249903
Study type:

combined biological and clinical study
Data availability: GSE63119; GSE46622; GSE43793; GSE32323
Code availability: -