circRNA basic information
circBase ID: hsa_circ_0000228
Name: hsa_circ_ZEB1
Synonym: ZEB1‐circRNA
Host Gene: ZEB1
Genomic location(hg19): chr10:31661946-31676195:+
Genomic location(hg38): chr10:31373017-31387266:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018881
MONDO name: myelodysplastic syndrome
Disease details: Myelodysplastic syndrome / MDS
Disease DO ID:
 0050908
Disease MeSH ID:
 -
Disease NCIt ID:
 C3247
Disease ICD11 ID:
 -
Disease OMIM ID:
 614286
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow CD34+ cells
Cell lines:

NALM6; K562
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: miR‐1248
Biological function: Regulates cellular metabolism/mitochondrial activity in SF3B1-mutated cells; its knockdown increases oxidative phosphorylation and reduces glycolysis.
Molecular mechanism: Binds (sponges) miR-1248, affecting its expression; associated with mitochondrial processes.
Biological pathway or process:

oxidative phosphorylation (inhibits); glycolysis (promotes); mitochondrial function (other); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Transfection; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

-
Description:

hsa_circ_0000228 (a ZEB1-derived circRNA) is strongly up-regulated in SF3B1-mutated MDS. Its knockdown alters cellular metabolism by increasing mitochondrial respiration/oxidative phosphorylation and decreasing glycolysis, and it directly binds miR-1248, consistent with a ceRNA-like mechanism linked to mitochondrial processes.
Confidence score:

0.6715
Other information
Title:

Expression of circular RNAs in myelodysplastic neoplasms and their association with mutations in the splicing factor gene SF3B1.

Journal: Molecular oncology
Published: 2023
PubMed ID: 37408496
Study type:

combined biological and clinical study
Data availability: BioProject ID PRJNA896500
Code availability: -