circRNA basic information
circBase ID: hsa_circ_0000018
Name: hsa_circ_DNAJC16
Synonym: circDNAJC16
Host Gene: DNAJC16
Genomic location(hg19): chr1:15860731-15863309:+
Genomic location(hg38): chr1:15534236-15536814:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

LUAD tissues; adjacent tissues; paired non-tumorous tissues; adjacent non-cancerous tissues
Cell lines:

A549; H292
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: CDKN1A, p21, eIF4A3, AGO2, DNAJC16, cyclin D1, CDK4, E-cadherin, N-cadherin, Vimentin, beta-catenin, phosphorylated GSK-3alpha/beta, phosphorylated STAT3
Associated microRNA: miR-93-5p
Biological function: Suppresses LUAD tumor growth, proliferation, colony formation, migration and invasion; induces G0/G1 cell cycle arrest; inhibits metastasis through EMT regulation.
Molecular mechanism: circDNAJC16 functions as a ceRNA sponge for miR-93-5p, relieving repression of CDKN1A/p21 to induce G0/G1 arrest and suppress proliferation; it inhibits migration and invasion through EMT regulation, with possible involvement of Wnt/beta-catenin and STAT3 signaling; eIF4A3 binds upstream flanking regions and regulates circDNAJC16 subcellular localization/nuclear retention.
Biological pathway or process:

ceRNA regulation (promotes); cell cycle (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); Wnt/beta-catenin (inhibits); JAK/STAT (inhibits)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Reduced circDNAJC16 expression correlates with advanced pathological stage, lymph node metastasis, elevated serum CEA and poor prognosis; it may serve as an independent biomarker for predicting LUAD patient outcomes.
Description:

circDNAJC16 (hsa_circ_0000018) is a human DNAJC16-derived circRNA downregulated in LUAD tissues, with low expression associated with advanced stage, lymph node metastasis and poor overall survival. Functionally, circDNAJC16 suppresses LUAD proliferation, tumor growth, migration and invasion by sponging miR-93-5p and activating CDKN1A/p21-mediated G0/G1 arrest, while also inhibiting EMT and potentially Wnt/beta-catenin/STAT3 signaling. eIF4A3 binds upstream flanking regions of DNAJC16/circDNAJC16 and regulates circDNAJC16 subcellular localization, supporting its role as a prognostic biomarker and therapeutic target.
Confidence score:

0.8981
Other information
Title:

EIF4A3-mediated localization of circDNAJC16 sequesters miR-93-5p to suppress lung adenocarcinoma progression via CDKN1A-regulated cell cycle and EMT.

Journal: Experimental cell research
Published: 2025
PubMed ID: 41138842
Study type:

combined biological and clinical study
Data availability: Supplementary information files; raw data available by authors without undue reservation; data available on request
Code availability: -