| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, NF-kappaB |
| Associated microRNA: |
- |
| Biological function: |
Promotes inflammatory response, proliferation, and migration of keratinocytes in AD. |
| Molecular mechanism: |
circRHOBTB3 interacts with RNA-binding protein EIF4A3 to upregulate and activate NF-kappaB signaling; NF-kappaB promotes transcription of circRHOBTB3, forming a positive feedback loop. |
| Biological pathway or process: |
NF-kappaB (promotes); inflammation (promotes); proliferation (promotes); migration (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Actinomycin D / DRB Stability Assay; Transfection; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; ELISA; Western Blot; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circRHOBTB3 is up-regulated in an IL-4-induced HaCaT cell model of atopic dermatitis and is predominantly cytoplasmic. Its knockdown suppresses inflammatory cytokine release, proliferation, and migration, while mechanistically circRHOBTB3 interacts with EIF4A3 to activate NF-kappaB signaling and forms a positive feedback loop with NF-kappaB transcriptional regulation. |
| Confidence score: |
0.7545 |