| Expression pattern: |
UP |
| Associated gene: |
circCHD6-216aa, DHX9, NCL, CD44 |
| Associated microRNA: |
- |
| Biological function: |
circCHD6 promotes CRC cell migration and invasion in vitro and promotes CRC metastasis in vivo; its encoded protein circCHD6-216aa functions as a pro-metastatic driver. |
| Molecular mechanism: |
circCHD6 encodes circCHD6-216aa, which undergoes LLPS, promotes DHX9 phosphorylation to enhance circCHD6 biogenesis in a positive feedback loop, and interacts with NCL to stabilize NCL phase separation and enhance CD44 transcription. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; RNA-seq; IF (Immunofluorescence); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; H&E Staining; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circCHD6 expression in CRC patients is associated with poorer prognosis and may be associated with tumor progression. |
| Description: |
circCHD6 is up-regulated in colorectal cancer and higher expression is associated with poorer patient prognosis. It encodes circCHD6-216aa, a pro-metastatic protein that forms nuclear LLPS condensates, enhances circCHD6 biogenesis through DHX9 phosphorylation, and stabilizes NCL phase separation to promote CD44 transcription and CRC metastasis. |
| Confidence score: |
0.9013 |